Metascreen® Testing platform

Metascreen® has been using advanced and FDA-approved GC-MS technology combined with proprietary bioinformatics to reliably detect more than 100 metabolic disorders. The laboratory has participated in and passed various external proficiency testing programs from College of American Pathologists and ERNDIM since beginning of 2015.

Comparison of Testing Platforms

Parameter GC-MS MS/MS
Newborn specimen collected Urine:
  • Contains more than 3,000 detectable metabolites, with many in higher concentration than in blood
  • Non-invasive, safe, and simple for collection by parents or hospital staff
Blood:
  • Though contains similar number of detectable metabolites as urine, many not in as high concentration than in urine
  • Invasive, painful, requires technical expertise, risk of infection
  • Certain metabolic disorders do not alter blood amino acid or acylcarnitine profile, hence further testing is required to differentiate type of disorder
No. of metabolic disorders ("IEMs")that can be detected and quantified 100+ IEMs from 9 groups of IEMs
  • Acid and organic acid metabolism disorders
  • Sugar metabolism disorders
  • Fatty acid metabolism disorders
  • Peroxisomal disorders
  • Purine & pyrimidine metabolism disorders
  • Lactic acid, hyperpyruvic acid metabolic disorders
  • Other IEMs
40+ IEMs from 3 groups of IEMs
  • Amino acidemia
  • Organic acidemia
  • Fatty acid metabolism disorders
Biomarkers, i.e. no. of analyte profiles used Multiple markers used:
Highly specific, definitive interpretation from one single test
Mostly single marker used:
Non-specific, ambiguous – 2nd tier testing required
Time to diagnosis Faster:
From screening-cum-confirmation to diagnosis
Slower:
From screening to confirmation then finally to diagnosis
Further confirmation required Screening and confirmation from one single test, though occasionally requires further confirmation via:
  • Enzyme assays
  • Genetic testing
Requires confirmatory test via:
  • GC-MS analysis
  • Enzyme assays
  • Genetic testing
Throughput Approximately 15 minutes per sample:
Total throughput of about 60 – 90 samples per machine in one day
Approximately 2 minutes per sample:
Total throughput of about 500 samples per machine in one day thus better for mass screening
Interference
  • Chromatography separates potential interfering compounds, such as drugs and exogenous chemicals
  • Acylcarnitine profiling not critical in GC-MS urinary analysis
Certain drugs and exogenous chemicals can interfere with acylcarnitine profile analysis e.g. valproic acid, cefotaxime, dextrose

Contact Us

Cordlife Technologies Pte Ltd
1 Yishun Industrial Street 1
#06-01/09 A'Posh Bizhub
Singapore 768160

Email
metascreen@cordlifetech.com